Identify the sections of the following segment representing Background, Purpose, Experimental approach, Results, and Interpretation of Results
Considerable evidence suggests that ATP is needed in the binding of mRNA to the 40S ribosomal subunits (13). To understand the interaction between ATP and mRNP (mRNA with bound proteins) particles better, we incubated the mRNP particles with 14C ATP at optimal concentrations for in vitro yeast translation. Results indicate that 14C ATP bound to mRNP particles, but the binding decreased about 4-fold when the temperature increased from 4 to 17° C (Fig. 1). These results suggest that the binding between ATP and mRNP particles may be governed by interactions such as hydrogen bonds or van der Waals that weaken when temperature rises.
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