Chapter 12 Exercises

. For medical studies include (select all that apply):

Total number of subjects

Number of subjects receiving treatment

How subjects were selected

Details such as sex and age if relevant

. Does the following example provide sufficient detail?
All samples were centrifuged.

Yes

No

. Does the following example include unnecessary detail?
CD4⁺ CD44^high T cell purification
We were interested in examining the gene expression of the anergic portion of T cells in Jak3 KO mice that are CD4⁺ CD44^high. To obtain the CD4⁺ CD44^high T cells used for RNA isolation, spleens were removed from Jak3 KO and Jak3 Het mice at 8 to 10 weeks of age. Splenocytes were isolated by homogenizing the tissue with frosted glass slides (Fisher Scientific, Pittsburgh, PA).

Yes

No

. Does the following example include unnecessary information?
Cells were scraped out of the wells and resuspended in a 1.5 ml Eppendorf tube.

Yes

No

. Which of the following are common problems of the Materials and Methods section? Select all that apply.

Lengthy purpose statement

Omission of the purpose for an experiment

Too many details

Insufficient details

. Select the better sentence.

Here we provide a description of how subjects were chosen for our study. We selected only healthy males between 60 and 80 years.

For our study, we selected only healthy males between 60 and 80 years.

. Select the better sentence.

Study subjects were presented with a list of potentially traumatic events, and we asked them to use three response categories (yes, no, unsure) to indicate if they had ever experienced them.

Study subjects were presented with a list of potentially traumatic events, and were asked to use three response categories (yes, no, unsure) to indicate if they had ever experienced them.

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